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Mid-Atlantic
Research Facility
(MRF)
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Internationally
recognized for its red cell and platelet studies Mid-Atlantic
Research Facility has conducted more than 200 research projects
and licensing studies for biomedical companies resulting in
over 140 scientific publications since its founding in 1978
and is recognized as a forerunner in basic and applied research
concerning red cell and platelet physiology, storage and function. |
The Mid-Atlantic Research Facility assists companies with licensing
studies for new products. This can include the development of protocols,
setting up, conducting, evaluating, and/or preparing final reports
for complex blood studies. The research staff has developed techniques
which serve as a standard for labeling and measuring viability and
survival of platelets and red cells. The department also has served
as a training center for these procedures.
The Mid-Atlantic
Research Facility is located in the Medical Tower Building of the
Eastern Virginia Medical School (EVMS) and Sentara complex, as well
as the Blood Isotope Laboratory in the Nuclear Medicine Department
of Sentara Norfolk General Hospital (SNGH). The staff also utilizes
facilities and equipment in SNGH's Nuclear Medicine Department,
as well as their Flow Cytometry laboratory. Access to modern molecular
biological techniques are available through a strong collaborative
relationship with the Molecular Biology Laboratory at EVMS. Coordination
between our laboratory and the American Red Cross, Mid-Atlantic
Regional blood Services' apheresis unit, nursing staff, and technical
laboratories is well established.
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Procedures
and assays in current use include: |
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Radioisotope
labeling methods for measurement of red cell mass, blood volume,
red cell and platelets recovery and survival using technetium-99m,
chromium-51, and indium-111 (Tc99m, Cr51, In111). |
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Concurrent
double label method for platelet survival studies. |
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Standard
enzymatic assays and adenine nucleotide measurement of red cell
biochemistry. |
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Morphologic
and functional tests of red cells (osmotic fragility, morphology). |
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Evaluation
of leukoreduced products (Nageotte counting, percent cell loss). |
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Standard
enzymatic assays for measurement of platelet biochemistry. |
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Morphologic
and functional platelet tests including: oxygen uptake, hypotonic
shock response, discoid shape evaluation by photometry, size
distribution (Coulter), aggregation (photometry, impedance,
counting), adhesion (everted aorta, collagen-coated beads). |
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Specialized
platelet tests: surface expression of glycoproteins (flow cytometry),
I125 complement assays, Beta thromboglobulin (BTG) using EIA |
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Plasma
analysis - protein assays, coagulation (factor assays) |
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Stem
cell and cord blood collection and evaluation |
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Evaluation
of different automated blood collection systems/technologies. |
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Pathogen
detection/reduction studies. |
MRF's facilities are in compliance with all requirements of the
Food and Drug Administration, the Nuclear Regulatory Commission,
OSHA, and American Red Cross Biological Safety directives. All studies
are reviewed and approved by the Institutional Review Board of Eastern
Virginia Medical School, and the Radiation Safety, Patient Care,
and Executive Committees of Sentara Norfolk General Hospital. Current
Good Manufacturing Practices are followed in performance of all
procedures, and the laboratory continues to meet the standards of
all sponsors as determined by on-site inspections.
The staff has access to an excellent donor pool (medical students,
hospital and blood bank personnel), which facilitates recruitment
of a well-informed, knowledgeable volunteer population. Well-established
coordination with regulatory approving agencies allows timely start-up
of projects. In most cases, research studies can be underway within
thirty (30) days.
Medical Towers Building
400 Gresham Drive, Suite 100
Norfolk, VA 23507
Phone:
757-446-7720
Fax: 757-533-5725
Email: whitleyp@usa.redcross.org
| Mid-Atlantic
Research Facility's Staff |
Pamela H. Whitley, M.S, MT(ASCP)SBB, Research Manager
Sherrie Sawyer, M.S, Senior Research Specialist
Mike Wellington, M.S, Research Associate
Valerie Vann, Administrative Assistant
Eldora Snowden, Research Collection Technician
The staff has
over fifty (50) years of cumulative research experience. They are
well trained in the use of in vitro and in vivo techniques, and
have extensive experience in radioisotope labeling procedures. They
perform evaluations of procedures and products concerning collection,
processing, and storage of platelets and red blood cells as required
by the FDA for licensing new products. Consultations of the development
of clinical trial are also available.
Join the list of our satisfied customers:
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Baxter
Healthcare Corporation |
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CaridianBCT, Inc. |
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Fresenius |
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Haemonetics
Corporation |
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Medsep
Corporation |
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PALL
Corporation |
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Terumo
Corporation |
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Kowa
Company, Ltd. |
"I was very impressed with our last study and will come back
to your laboratory when I have other
studies to do!"
-Michael McAteer, Ph.D. Therapy Scientist. Gambro BCT, Inc.
"It is a pleasure working with such experienced individuals.
The excitement and energy in which your team tackles a project,
assures me that the results will be top notch and the turn around
time will be exceptional."
-Stein Holme, Ph.D. Pall Corporation
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